Effects of Chronic Nicotine Administration on Salivary Gland in Mice: Immunohistochemical Study
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Á¤Á¤¿ì ( Jung Jung-Woo ) - Kyungpook National University School of Dentistry Department of Oral Medicine
ÃÖÀç°© ( Choi Jae-Kap ) - Kyungpook National University School of Dentistry Department of Oral Medicine
º¯Áø¼® ( Byun Jin-Seok ) - Kyungpook National University School of Dentistry Department of Oral Medicine
KMID : 0355220140390030083
Abstract
Purpose: Chronic nicotine administration induce various effects in whole organs of the body; however, little is known about salivary gland. In the present study, we pursued the links between systemic nicotine and the histomorphological changes of the salivary gland in mice.
Methods: Twenty-five C57BL6 mice were allocated into two groups. The control group (n=9) received distilled water only for 8 weeks by gavage. The experimental nicotine group (n=16) was administered nicotine 5 ¥ìg/g with distilled water. Animals were sacrificed at 8 weeks; then, submandibular glands were excised and processed for histologic evaluation. Volumetric changes in acinar cells were evaluated by H&E staining. The expression of calponin-positive myoepithelial cells and Ki-67-positive proliferating acinar cells were evaluated by immunohistochemistry.
Results: The nicotine group showed significantly decreased number of calponin-positive myoepithelial cell process compared with the control group. There were no significant differences in average volume of acinar cell and the number of Ki-67-positive acinar cells between both groups.
Conclusions: These findings suggested that chronic nicotine administration may cause decreased function of myoepithelial cells in submandibular glands of mice, and these can partly explain xerostomic conditions in chronic smokers.
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Acinar cells; Calponin; Myoepithelial cells; Nicotine; Submandibular gland; Xerostomia
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